CONFIRMING LOCATION OF NITROGEN FIXING GENES ON PLASMIDS IN RHIZOBIUM ISOLATED FROM PISUM SATIVUM

To confirm the location of the nitrogen fixing genes whether on the plasmids or on the genomic DNA the Rhizobial isolates isolated from pea (Pisum sativum) were treated with acridine orange with various concentrations and fixed nitrogen was estimated from the media in which these rhizobia were grown. There was no significant difference in between the cured and non cured strains of the Rhizobium which proved that the nitrogen fixing genes are not plasmid borne but these are located on the genomic DNA.