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Studies of genetic polymorphism in the isolates of Fusarium solani

Keywords: Fusarium solani , Carboxylesterase , RAPD-PCR

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Abstract:

Fusarium solani is the important pathogen causing wilt disease of guava in India. In the present investigation six representative isolate of Fusarium solani, collected from different places of India were subjected to analysis of genetic variability in terms of Carboxylesterases isozyme pattern and DNA polymorphism using RAPD-PCR. Pattern of Carboxylesterase revealed a similar isozyme cluster in the isolate namely, Allahabad (isolate-3), Faizabad, (isolate-4), Unnao (isolate-5) and Lucknow (isolate-6). Similar results were obtained when 10 randomly amplified polymorphic DNA markers (OPA1-OPA10) tested in the genome of Fusarium solani and grouped on basis of obtained allelic data. This pattern of genetic variability in the isolate was also supported by the analysis of the similarity indices and UPGMA dendrogram.

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