ARAUCARIA EXCELSA L. VITROCULTURES INITIATION

Araucaria excelsa L. is a well-known conifer, mostly used as an indoor ornamental plant. For the initiation of Araucaria excelsa L. vitrocultures, we have studied the reactions of explants, in the presence of different growth regulators, added in the aseptic nutritive media. We have prelevated apexes from a unique plant and used them as biological material. The explants were sterilized and inoculated on BM media, with and without growth regulators. This experiment, which lasted for 90 days, has brought forth the following conclusions: On V0 (control variant– BM without growth regulators), the inoculs have presented a very week regenerative capacity; the best medium for elongation of Araucaria excelsa L. was V2 (BM with 2 mg/l BA + 2 mg/l NAA); the most ramifications and buds can be obtained if using V4 experimental variant (BM with 0.5 mg/l NAA + 0.5 mg/l KIN); at any experimental variant the rhysogenesis wasn’t observed.