MORPHO-ANATOMICAL AND PHYTOCHEMICAL RESEARCHES REGARDING PSEUDOTSUGA MENZIESII (MIRBEL) FRANCO (PINACEAE)

The paper presents morpho-anatomic and phytochemical researches of needles and young shoots of Pseudotsuga menziesii (Mirbel) Franco in Romania. The composition of needle and young shoots essential oils in Douglas-fir needles from different population are studied for the first time. Essential oil extraction of P. menziesii was performed using a neo-Clevenger-type apparatus and the analysis procedures with a GC-MS system. The major constituents of the oils were found to be sabinene, terpinolene, terpinene 4-ol, β-pinene, α-terpinen, β-phellandrene and α-pinene in various chemical concentrations due to analyzed vegetative organs (needles / young shoots), ecological conditions (chemotypes), analytical methods or plant phenophases.