SOMATIC EMBRYOGENESIS IN RUBUS CAESIUS L. SUSPENSION CULTURES

In vitro cultures technologies include many applications: plants improvement, micropropagation, unconventional alternatives to exploit secondary metabolites. The regenerative potential of Rubus caesis L. was evaluated for the establishment of a propagation protocol. Not only the capacity of differentiation was tested, but also the regenerative potential. The initiation of in vitro cultures at Rubus sanctus Schreb was achieved from axillary buds, cultivated on different variants of Murashige-Skoog medium. The callus cultures obtained by axillary buds dedifferentiation were multiplicated on agar medium and liquid medium. The MS medium supplemented with 1mg/l benzylaminopurine and 0,1 mg/l IAA stimulated somatic embryogenesis at Rubus caesius L. suspension cultures ,whereas the MS medium supplemented with 1mg/ kinetine generated an intensive proliferative reaction and callus development. The regeneration of whole plants was obtained in three steps: callus initiation, suspension cultures initiation, somatic embryogenesis induction.