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MORPHOLOGICAL CHARACTERIZATION AND THE GERMINATING POTENTIAL OF TRIFOLIUM MONTANUM L. AND T. PANNONICUM JACQ. POLLEN

Keywords: pollen grain , exine ornamentation , germinating potential of pollen

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Abstract:

The paper presents the general morphological aspects of Trifolium montanum L. and T. pannonicum Jacq. pollen with the diagnosis value of the respective taxa. It also presents the variability of pollen germinating capacity in the two taxons from the Fabaceae family, according to the factor of glucidic concentration in the artificial germination environment. We have tested germination in nutritive mediums lacking glucidic elements and in mediums with different sucrose concentrations, until 350%. This paper presents the concentration of glucidic elements from mediums allowing pollen germination at minimum percentage, shows the sucrose concentrations for developing the germination process under best conditions and analyses the germination process in dynamics (after 3, 24, 48 and 72 hours since pollen inoculation in nutritive mediums). The obtained results may be correlated to the amplitude of the variability in pollen grain size, to the ecological plasticity typical of the two taxons, plasticity controlled by the genetic substratum typical of the two tightly related species. It was shown that the pollen germinating potential was different, being a species trait, with diagnosis value. The Trifolium montanum pollen having a higher germinating potential, it could be correlated to the vast spreading area of this species, which is represented in europe, caucasia, siberia, persia, america.the germinating potential of the Trifolium pannonicum pollen (with spreading area in europe and in north of northern of america), lower than of the other taxon, could be explained by the high polyploidy degree.

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