ASPECTS OF “IN VITRO” CULTIVATION OF CHELIDONIUM MAJUS L.

The initiation of “in vitro” cultures of Chelidonium majus L aimed not only to assess the dedifferentiation capacity depending on explant origin and growth regulators, but also to develop a multiplication protocol based on direct regeneration from shoots explants, followed by roots development induction.The proliferative capacity was tested on leaf and shoots explants, cultivated on Murashige-Skoog basal medium, testing two auxins: 2.4 diclorphenoxiacetic acid (2,4D) and indolylacetic acid (IAA) in combination with a cytokinine: kinetine (K). The biomass accumulation was measured by regular weighing on analytical balance. Regenerative capacity was evaluated on shoots explants, cultivated on different inductive variants of MS medium. Benzylaminopurine (1 mg/l) and in combination with 2.4 diclorphenoxiacetic acid (0,5 mg/l) stimulated growth and multiplication of shoots. Root system development was achieved on MS medium without growth regulators.