Micro-propagation and 1 , 2-didehydrostemofoline Production from Stemona Sp.

Plant propagation through tissue culture technique is required to produce plant secondary metabolite. The objective of this experiment was to obtain the optimum media composition for in vitro shoot and root formation of Stemona sp. It was shown that Murashige and Skoog (MS) medium supplement with 3 mg L-1 benzyladenine and half-MS medium supplemented with 2 mg L-1 indolebutyric acid were the appropriate media for shoot and root induction, respectively. The media could produce 100% of shoot induction and 80% of root induction. The increase or decrease in 1 , 2-didehydrostemofoline was related to the root growth. The highest 1 , 2-didehydrostemofoline production of 31.04 mg g-1 root dry weight was observed in 16 week old root.