Resolving Genetic Relationships Among Aegilops L. and Triticum L. Species Using Analysis of Chloroplast DNA by Cleaved Amplified Polymorphic Sequence (CAPS)

Studying of genetic relationships among Triticum L. and Aegilops L. species by using Cleaved Amplified Polymorphic Sequence (CAPS) to investigate the polymorphism in chloroplast DNA (cpDNA) among 20 Aegilops L. and 7 Triticum L. species and one accession of Amblyopyrum muticum. 23 chloroplast regions were amplified then Restriction digests were performed on PCR amplicons instructions using 72 singlecut Endonucleases. The rseults indicate that there are 16 accessions from Triticum and Aegilops species have positive and unique restriction profiles in 5 loci. Inaddition 11 accessions from Aegilops and one accession of Amblyopyrum muticum have identical restriction profiles in all loci.