ENRAIZAMENTO IN VITRO DE RAMOS DE MAMOEIRO ‘TAINUNG 01’ CULTIVADOS SOB LUZ e ESCURO/LUZ

This work aimed to evaluate two culture conditions in vitro in rhizogenic response of micropropagated branches of papaya tree (Carica papaya L. 'Tainung 01'). The branches submitted to rooting were obtained in the fourth subculture in the midst of multiplication and cultivated in culture medium of Murashige and Skoog (1962), supplemented with sucrose at 30 g L-1, and IBA at 0.2 mg L-1solidified with agar at 7g L-1. Were used 40 branches but 20 of these branches were for 7 days starting in the dark and a further 23 days under fluorescentlight in providing 22.8 μmol m-2s-1flows of photosynthetic photons and 16 hours of photoperiod and temperaturecontrolled 27 2oC. The other 20 branches remained under light for 30 days under specified conditions. At the end of this period were evaluated the percentage of rooted branches, the growth of the branches, the length of the largest leaf and the mass of callus at the base of the branches. The means were analyzed by t-test at 5% probability. By the results obtained only for the variable mass of callus was statistically significant difference being that there was more callus formation at the base of branches grown in the dark for seven days early. It is concluded that the roots using IBA at 0.2 mg L-1can be done under light or dark / light. However, for reasons of energy saving, it is recommended that the culture condition in the dark / light.Esse trabalho teve como objetivo avaliar duas condi es de cultivo na resposta rizogênica in vitro de ramos micropropagados de mamoeiro (Carica papaya L. ‘Tainung 01’). Os ramos submetidos ao anraizamento foram obtidos no quarto subcultivo em meio de multiplica o e cultivados em meio de Murashige e Skoog (1962), suplementado com sacarose a 30 g L-1, e AIB a 0,2 mg L-1solidificado com 7 g L-1de Agar. Foram usados 40 ramos sendo que 20 destes estiveram por sete dias iniciais no escuro e mais 23 dias seguintes na luz sob lampadas fluorescentes fornecendo 22,8 μmol m-2s-1de fluxos de fótons fotossintéticos e 16 horas de fotoperíodo com temperatura controlada em 27 2oC. Os outros 20 ramos estiveram todos os 30 dias sob luz nas condi es especificadas. Ao final deste período foram avaliados a porcentagem de ramos enraizados, o crescimento dos ramos, o comprimento da maior folha e a massa de calo na base dos ramos. As médias foram analisadas pelo teste t a 5% de probabilidade. Pelos resultados obtidos apenas para a variável massa de calo houve diferen a estatisticamente significativa sendo que há maior forma o de calo na base de ramos cultivados no escuro por sete dias iniciais. Conc