Delignification of Rice Straw with Ligninase from Novel Penicillium sp. strain apw-tt2 for Biopulping

Study of biodelignification of rice straw wasinvestigated by using a fungus isolated from indigenous thestraw. Identification of the fungus was characterized by 18srRNA sequencing and included in the new species ofPenicillium sp. strain apw-tt2. Ligninase production and cellgrowth was observed in incubation condition of SubmergedFermentation (SmF). Optimizations of the enzyme productioncondition were carried out by altering pH and temperature ofthe incubation medium. The results showed that the optimumconditions for biopulping are at 40 C and pH 5. High ligninaseproduction was observed at Day 4 with activity 571 U/ml withdegraded lignin 66.3%. The quality of pulp showed that the newspecies is very effective in delignifying rice straw for pulping.