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Molecular phylogenetic study of Luffa tuberosa Roxb. (Cucurbitaceae) based on internal transcribed spacer (ITS) sequences of nuclear ribosomal DNA and its systematic implication

Keywords: Luffa tuberosa , Momordica , Cucurbitaceae , ITS , nuclear ribosomal DNA

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Abstract:

The phylogenetic position of long been debatable species Luffa tuberosa was inferred in thepresent study using ITS sequence of nuclear ribosomal DNA data. The study sampled a total number of 16accessions which include five accessions of Luffa (under four species i.e. Luffa acutangula, L. cylindrica, L.aegyptiaca and L. tuberosa), nine accessions of Momordica (under eight species i.e. M. angustisepala, M.balsamina, M. cabraei, M. charantia, M. charantia subsp. macroloba, M. cissoides, M. cochinchinensis, M.dioica and M. foetida) and two accessions of Trichosanthes under two species (i.e. T. lepiniana and T.tricuspidata). The sequence data analysis clearly reveals nesting of Luffa tuberosa within the clade ofMomordica, thus, we herein support the inclusion of Luffa tuberosa into the genus Momordica as M.tuberosa (Roxb.) Cogn.

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