HPTLC Fingerprinting in the Standardization of Panchavaktra Ras: A Herbo-Mineral Preparation

High performance thin layer chromatography (HPTLC) is an enhanced form of thin layer chromatography (TLC). A number of enhancements can be made to the basic method of thin layer chromatography to automate the different steps, to increase the resolution achieved and to allow more accurate quantitative measurements. Automation is useful to overcome the uncertainty in droplet size and position when the sample is applied to the TLC plate by hand. One recent approach to automation has been the use of piezoelectric devices and inkjet printers for applying the sample. The assay combines the separation and quantification of the analyses on silica gel HPTLC plates with visualization under UV and scanning. Using this technique, the alkaloid content of different parts of the drug Panchavaktra ras finger print profile has been determined for the 3 experiments in the different times. HPTLC of Panchavaktra ras is the preliminary quantitative analysis which shows the number of components present in the sample accurately and precisely on the basis of mild variations in Rf values, that can acceptable in this drug and it indicates the purity of drugs. The UV spectrum of the common peaks with large abundance appeared at Rf 0.46, 0.33, 0.28, 0.30, 0.41, 0.49, 0.56, 0.57, 0.59, 0.99 were found to be super imposable revealing the presence of the same constituents in all the 3 samples.