Assay of Ozone Acute Toxicity Applied by Autohemotherapy to Rats

Ozone (O3) with its great oxidant power is able to read with biological molecules which are available in the blood such as membrane phospholipids and in this way are produced oxidized substances which are able to produce therapeutic effects, such as to restore the antioxidant/prooxidant balance and in this way modulate the inflammatory response. With the aim in order to evaluate the potential toxic effect of O3 applied by autohemotherapy, male and female SD rats were used, which were divided in four experimental groups of 16 rats (8 per each sex). Group 1 received treatment with Oxygen, group2 treated with O3 (21.12 μg/mL, doses 168 μg/kg); group 3, treated with O3 (47.04 μg/mL, doses 376 μg/kg); group 4, treated with O3 (64.16 μg/mL, doses 512 μg/kg). For O3 application, 2 mL of blood which are mixed with 2 mL of O3 to the concentration before described for each group. This mixture is injected by the tail vein only once. Thereafter the rats were observed during 14 days and were registered the clinical signs and body weight of the rats. Thereafter, the rats were killed and the state of the organs was evaluated. The rats showed a normal behavior and no signs of toxicity were found. In conclusion, we found that applied O3 does by autohemotherapy were non toxic.