Antibacterial Potentials of Bark and Leaves Extracts of Juglans Regia against Antibiotic Resistant Bacteria

Plant parts were collected from Abbottabad District, Khyber Pakhtunkhwa. Then crude methanolic extract of bark and leaves of Juglans regia were taken. Each methanolic crude extract was diluted in Dimethyle sulphoxide (DMSO) up to the concentration of 60 mg/ml, 90 mg/ml and 120 mg/ml. The plant parts were examined for antibacterial activity against the gram positive bacteria (Staphylococcus aureus) and gram negative bacteria (Agrobacterium tumefaciens). Juglans regia bark and leaves show remarkable antibacterial activity against gram positive and gram negative bacteria. By comparing the results with the standard antibiotics lincomycine and ceftrixone, both standards have greater zone of inhibition against the gram positive and gram negative bacteria. From both the plant extracts as well as antibiotics, it was found that the gram positive bacteria (Staphylococcus aureus) was more sensitive than gram negative bacteria (Agrobacterium tumefaciens). Thus, the plant extract of Juglans regia bark was found to have more potent antimicrobial activity against gram positive bacteria, Staphylococcus aureus, which can be used to treat skin diseases.