Cancer-associated fibroblasts are positively correlated with metastatic potential of human gastric cancers

Immunochemistry and realtime-PCR experiments were carried out to compare the expression of proteins which are specific markers of CAFs or secreted by CAFs in the tumor and normal tissue specimens. The extent of CAFs' prevalence was graded according to immunochemical staining, and correlation was further analyzed between CAFs' prevalence and other tumor characteristics which may influence the prognosis of gastric cancer patients.Nearly 80 percent of normal gastric tissues were negative or weak positive for CAFs staining, while more than 60 percent of gastric cancer tissues were moderate or strong positive for CAFs staining. Realtime-PCR results also showed significant elevated expression of FAP, SDF-1 and TGF-β1 in gastric cancer tissues compared to normal gastric tissues. Further analysis showed that CAFs' prevalence was correlated with tumor size, depth of the tumor, lymph node metastasis, liver metastasis or peritoneum metastasis.Reactive cancer associated fibroblasts (CAFs) were frequently accumulated in gastric cancer tissues, and the prevalence of CAFs was correlated with tumor size, depth of the tumor and tumor metastasis, thus give some supports for establishing the prognosis of the gastric cancer patients.Gastric cancer is the second leading cause of cancer-related death worldwide [1]. Substantial geographic variations exist in the incidence of gastric cancer and it represents the most common cancer in China [2]. More and more gastric cancer patients have been diagnosed in recent years with changing diet and lifestyle as well as developing diagnostic procedures. Although surgical treatment has shown to be effective for some early gastric cancers, including total gastrectomy and extended radical gastrectomy, the prognosis of these patients is poor due to the recurrence after surgery, in the form of lymphatic spread, blood-borne metastasis, or peritoneal dissemination [3].The prognosis of patient with gastric cancer has been shown to be influenced by several e