NDRG2 suppresses the proliferation of clear cell renal cell carcinoma cell A-498

The NDRG2 recombinant adenovirus plasmid was constructed. The proliferation rate and NDRG2 expression of cell infected with recombinant plasmid were mesured by MTT, Flow cytometry analysis and western blot.The CCRCC cell A-498 re-expressed NDRG2 when infected by NDRG2 recombinant adenovirus and significantly decreased the proliferation rate. Fluorescence activated cell sorter analysis showed that 25.00% of cells expressed NDRG2 were in S-phase compared to 40.67% of control cells, whereas 62.08% of cells expressed NDRG2 were in G1-phase compared to 54.39% of control cells (P < 0.05). In addition, there were much more apoptotic cells in NDRG2-expressing cells than in the controls (P < 0.05). Moreover, upregulation of NDRG2 protein was associated with a reduction in cyclin D1, cyclin E, whereas cyclinD2, cyclinD3 and cdk2 were not affected examined by western blot. Furthermore, we found that p53 could upregulate NDRG2 expression in A-498 cell.We found that NDRG2 can inhibit the proliferation of the renal carcinoma cells and induce arrest at G1 phase. p53 can up-regulate the expression of NDRG2. Our results showed that NDRG2 may function as a tumor suppressor in CCRCC.Renal cell carcinoma (RCC) accounts for 3% of all malignant tumors and 90% of neoplasms arising from the kidney. The incidence rates vary more than 10-fold around the world; rates are higher in Western countries than in Asia. In the United States, renal cancer is the 7th leading malignant condition among men and the 12th among women [1]. Clear cell renal cell carcinoma (CCRCC) originates from proximal tubule cells and is the most common pathological type of renal cell carcinoma. Multiple genetic changes have been found in CCRCC, but little is known about major tumor suppressor genes involved in the tumorigenesis of the disease.N-myc downstream regulated gene 2 (NDRG2) belongs to the NDRG family, which is comprised of 4 members, NDRG1-4, and is expressed in the tissues of the brain, heart, skeletal muscle,