In vitro propagation of Aloe barbadensis Miller, a miracle herb

Aloe vera has valuable medicinal properties and is commercially used inpharmaceutical, cosmetic and food industries. An efficient micro propagation method hasbeen developed in Aloe vera plants using the shoot tip explants cultured on MS mediumwith different phyto hormonal supplements for shoot proliferation and rooting. Theshoot proliferation was found best (80%) in the MS medium containing Benzyl aminopurine (BAP) 2.0 mg/L. Seventy percent of adventitious root formation was observed inhalf strength MS medium supplemented with Indole butyric acid (IBA). After two weeks,in vitro grown plants were transferred to the poly-cups containing 1:1 ratio of soil andsand respectively for hardening and then transferred to garden showed 75% of survival.