In vitro callus induction in rice (Oryza sativa L.)

In vitro callus induction from embryos of matured seeds of four rice varieties viz.,ASD 16, ADT 43, Basmati 370, Pusa Basmati and Pokkali were studied. Observations oncallus induction were carried out on six different callus induction medium having differentconcentration of 2,4-D viz., 0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 mg/l. The callus induction frequencyvaried from 58.33 % to 96.67 %. This study will be useful for selecting suitable callusinduction medium for callus induction.