Analysis of interaction between Arbuscular Mycorrhizal fungi and their Helper bacteria by MILPA model

Many recent researches carried out the research in mycorrhizal symbiosis to improvecrop growth in the agriculture but they differ in their approaches like crops and theagricultural perspectives. The study focused on the interaction of Arbuscular mycorrhizal(AM) fungi and helper bacteria, influence maize growth through milpa as a model system toanalyze soil enzyme activity of acid, alkaline phosphatase and signaling molecule ofphospholipids fatty acid (PLFA) profile. Bioinoculants plays an important role in plantgrowth like nutrient mobilization, biocontrol and prevent the plants from stress. The maizecrops were treated with bio inoculants such as Azospirillum, Azotobacter, Rhizobium,Pseudomonas and AM fungi. Significant diverse effects were observed with bioinoculants inthe crops compared to control crop. The number spores formed was proportional to the rateof colonization.AM fungi association in plant roots helps the plants in nutrient uptakeespecially phosphorus (P) from soil materials. The enzyme activities were also found toinfluence the growth of the plant and phospholipids fatty acid (PLFA) profile influences theinteraction between bacterium and AMF. Plant and phospholipids fatty acid analysis is asensitive and accurate method in determining microbial community structures, because itdepends on living cell contents of microorganisms under in situ conditions. It confirmedwith gas chromatographic analysis of PLFA to determination of the structure and totalbiomass of microbial community in treated soil samples.