High-protein diet and high-grade proteinuria aggravate the progression of chronic renal failure in rats

Objective: To study the effects of high-protein diet (HPD) and high-grade proteinuria in aggravating the progression of chronic renal failure (CRF) in rats.Methods: CRF with high-grade proteinuria was induced by supplying HPD in five sixth nephrectomy rats, and the changes of serum creatinine (Scr), blood urea nitrogen (BUN), endothelin-1 (ET-1), thromboxane B2 (TXB2) and 6-keto-prostaglandin Flα (6-Keto-PGFlα) were observed. At the same time, the content of malondialdehyde (MDA), and the activities of glutathione peroxidase (GSH-Px) and superoxide dismutase（SOD）were measured in blood and renal tissue of the rats.Result: HPD and high-grade proteinuria could accelerate the damage of kidney through increasing the levels of ET-1 and TXB2, reducing the level of 6-Keto-PGFlα, and attenuating the activities of SOD and GSH-Px.Conclusion: HPD can accelerate the damage of kidney through inducing the high-grade proteinuria in five sixth nephrectomy rats, influencing the expression of kidney vasoactive substance, and reducing the anti-oxidation.