RECHERCHE DES BACTERIES LACTIQUES ET ETUDE IN VITRO DE LEURS PROPRIETES PROBIOTIQUES

The bacteria mainly used as potentials probiotic belong to the group of the lactic bacteria. In order to fulfill this function, these bacteria must answer a certain number of properties (antagonistic activity, tolerance to acidity, capacity of adhesion etc). In this study, 6 strains: Enterococcus faecium, d’Enterococcus faecalis, Lactococcus lactis ssp lactis, Lactobacillus collinoides, Lactobacillus delbrueckii ssp delbrueckii and Lactobacillus fermentum, have been isolated and characterized. Our data revealed that these strains were susceptible to most used antimicrobial drugs, have an antibacterial activity against pathogenic strains: (Listeria monocytogenes ATCC 19115, Staphylococcus aureus ATCC 25923, Staphylococcus epidermidis CIP 106510, Enterococcus faecalis ATCC 9212, Micrococcus luteus NCIMB 816, Salmonella typhimurium NRRLB 4420, Pseudomonas aeruginosa ATCC 27853 and Escherichia coli ATCC 35218). The tested strains showed a aptitude to produce acidity, and tolerated the bile Oxgall 0,3% and pH 3. Adherence assay to polystyrene revealed that these strains are fairly adherent. The studied strains present in vitro interesting probiotic properties (inhibiting effect, production of acids...) independently of their biotope.