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Characterization of porcine β-casein G allele (CSN2G)Keywords: β-casein , CSN2G , G allele , porcine milk proteins , polymorphism. Introduction. The milk of almost all livestock species contains Abstract: Following the sequencing of porcine (Sus scrofa) β-casein cDNA we have identified a new alleleat this locus (CSN2). Our sequence (GenBank accession number GU827390) differs from the sequencereported by Alexander and Beattie (X54974) in the following positions: 294, 443, 468, 647, four SNPsbeing observed. Because of the guanine in position 294 differentiating the newly found allele from allother previous sequences, we have chosen to name this allele “G”. This is in fact a silent mutation, boththe codons CCT and CCG respectively (position 83 in the mature protein) encoding the same amino acid(Pro). Taking in consideration all the differences found at DNA level, the protein derived from thenucleotide sequence of G allele is unique (there are no 100% similarities when compared to all otherknown protein sequences). For future references we propose, for this allele, the nomenclature: CSN2G.
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