Effect of Nanosilver Particles on Alanin Amino Transferase (ALT) Activity and White Blood Cells (WBC) Level in Male Wistar Rats, In Vivo Condition

Background: Nanosilver is extensively used in medical equipment. Given the importance of white blood cells (WBC) in the immune system and liver cells in the body detoxification, this study has examined the effects of nanosilver particles on the white cell count and ALT liver enzyme in male Wistar rats. Materials and Methods: This experimental study was conducted on 40 adult male Wistar rats, provided from Pasteur Institute of Tehran. Nanosilver particles with concentrations of 50, 100, 200 and 400ppm were intraperitoneally injected to the treatment groups. Then, the mean of ALT enzyme concentration and white blood cell count of rats were compared in case and control groups at day 12 after treatment through ANOVA test.Results: The greatest effect of nanosilver particles on the ALT liver enzyme of rats was at concentration 50ppm after 3 days of injection (p=0.002). Also the greatest effect of nanosilver particles on white blood cells of rats was at concentration 400ppm after 12 days of injection (p=0.0001).Conclusion: The oxidative stress induced by intraperitoneally injection of nanosilver particles in rats causes severe irritation and immune system and increases the creation of white blood cells and liver damage in rats treated with nanosilver particles.