Potential Anticancer Activity of Acanthus ilicifolius Extracted From The Mangroves Forest of Karwar, West Coast of India

Screening of different extracts and fractions from the plant Acanthus ilicifolius (Acanthaceae) has been conducted using the in - vitro comet assay for anticancer using Hela and KB Cell lines. The air dried whole plant (Leaf, Fruit and Roots) was sequentially extracted into n - hexane, chloroform and methanol. The extracts were fractioned by column chromatography method and fractioned with ethyl acetate, acetone and water. All the extracts and fractions were tested for anticancer by comet assay. Ethyl acetate extract of whole plant showed significant anticancer activity. In conclusion, the ethyl acetate extract obtained from the whole plant of Acanthus ilicifolius showed a good cytotoxic effect to methanolic extract against Hela cells by in vitro method.