Research concerning the reactions of Sequoia sp. plantlets under culture, laid in horisontal and vertical position, on culture medias, with and without growth regulators

In this study I looked to the reactions of Sequoia plantlets under cultivated in vitro”, on different culture medias, laid in horizontal and vertical position, with and without growth regulators. This experiment has used apexes, the first under apical node and the basal node, taken from an old Sequoia in vitro” culture, as biological material. The experiment, which lasted for 90 days, has brought forth the following conclusion: the most efficient culture medium, from the four different tested variants, seems to be V1. In this medium the auxin had a huge effect on the callus development and the start of branching process of these plantlets. Also, the horizontal laid explants developed at a faster rate than the vertical ones.