In vitro reactivity of Begonia semperflorens cv. ‘Ambassador’ white to growth regulators

The objective of this experiment was to investigate the regeneration potential of a Begonia semperflorens cv. ‘Ambassador’ White cultivated on Murashige-Skoog standard mineral medium, supplemented with different combinations of growth regulators. After 90 days of in vitro culture, multiple shoots with 13.3 mm diameter with 12.5 leaves over 6 mm in diameter and with 4-5 buds, were efficiently obtained from the subcultured small propagules on variant V2 - mineral basic medium culture MB - MS supplemented with 1.5 mg / l thidiazuron. Callus induction was favored only on experimental variant V3 - mineral basic medium culture MB - MS supplemented with a mix of 1.5 mg / l 3-Indolebutyric acid plus 1.5 mg / l thidiazuron, with highest diameter of callus of 9 mm.