The effect of the in vitro environmental condition on organogenetic potential of two intergeneric hybrids Fragaria x Potentilla

Using liquid culture medium provided with filter-paper bridges, a simple plant regeneration system via organogenesis from leaf and petiole explants of two intergeneric hybrids Fragaria x Potentilla, named ‘Pink Panda’ and ‘Serenata’, has been developed. The regeneration capacity of the explants was influenced by the light condition and plant growth regulators concentration. Dark incubation during the first 6 weeks, followed by the transfer of the cultures under a photoperiod of 16 hours light/8 hours darkness, at a relatively low intensity of light was the most effective condition to successfully induce shoots regeneration in both intergeneric hybrids. The pretreatment darkness of 21 days, followed by the cultures transferring under a photoperiod of 16 hours light/8 hours darkness and a light intensity of about 40 μmol m-2 s-1 has been associated with an incapacity of calluses to form adventitious buds, regardless of the explant type or hormonal balance. Optimal shoot regeneration was obtained with BAP in a concentration of 3.0 mg/l, in combination with 1.0 mg/l IBA, added to modified Murashige-Skoog medium. Shootregeneration frequency were as high as 54.66% in ‘Serenata’ genotype and 43.33% in ‘Pink Panda’ genotype.