Studies regarding the chromatographic separation of some Chelidonium majus L. alkaloids using different solvent systems

Our study focuses on the investigation of a methanolic extract obtained from the roots of Chelidonium majus L. using the thin layer chromatography and different solvent systems in order to obtain the better resolution. The mobile phases that we used were represented by volumetric mixtures of methanol: chloroform: water (51:42:7), chloroform: methanol (7:3), chloroform: methanol (7:3) saturated with water, methanol: water (9:1), methanol: water (7,5:2,5), methyl-ethyl-ketone: water: methanol (67:30:3), chloroform: ethanol (35:1), chloroform: acetone: ethanol: toluene (10:5:5:5), 0,1% hydrochloric acid, chloroform: methanol: water (26:14:3) and n-propanol: water: formic acid (90:9:1). The last experiment was represented by a two dimensions thin layer chromatography with methanol: chloroform: water (51:42:7) as first dimension mobile phase and butanol: concentrated acetic acid: water (10:1:3) as second dimension mobile phase. The better resolution was obtained using the methanol: water (7,5:2,5), the methyl-ethyl- ketone: water: methanol (67:30:3) and the n-propanol: water: formic acid (90:9:1) mobile phases for one dimension TLC. The two dimensions TLC shows the separation of a big number of compounds that normally are not separated.