The influence of treatment with some purinic derivates on enzyme activity at Nicotiana tabacum L. plantlets

This paper present the influence of length treatment with two purinic derivates, on the three antioxidant enzyme activity: catalase(EC 1.11.1.6), peroxidase(EC 1.11.1.7) and superoxide dismutase(EC 1.15.1.1) in Nicotiana tabacum L plantlets. The substances using for this treatment was 1,3,7 – trimetilxanthine (teine, caffeine) and 1,3 – dimetilxanthine (theophylline), both with purinic nucleus in their structure. The treatment was applied twenty one days. In this period the plantlets was treated with these substances in following concentrations: 0.025%, 0.05%, 0.1% and 0.25%. In the same time was constituted and a control probe with plantlets treated only with distilled water. The treatment have determined the intensification of peroxidase and catalase activity and decrease of superoxide dismutase activity.