Low frequency low intensity pulse electromagnetic filed in vivo influence on blood cells permeability in rat

The in vivo influence of a low frequency and intensity (50 Hz, 2.7 mT) pulse electromagnetic field (PEMF) applied continuously and intermittently, 30 min for a period of 20 days, on the permeability of blood cells in rats was studied. For this aim, concentrations of Na+, K+ and Ca2+ in plasma (extracellular compartment) and blood cells (intracellular compartment) after their separation by centrifugation of blood collected from treated animals were determined by the flame photometry method. PEMF induced a decrease of the extracellular Na+ concentration and an increase of the intracellular one, causing a reduction in the ratio Na+e/Na+i value as compared with the control, reflecting the increase of passive membrane permeability to this ion. Also, PEMF induce the reduction of extracellular K+ level and increase the intracellular K+ level and a consequent K+e/K+i ratio reducing, which indicates an increase in transmembrane K+ active transport, correlated with dynamic of Na+,K+-ATPase activity. PEMF effect on Ca2+ is more diverse, manifesting a general trend of increasing of its intracellular concentration. These findings also indicate a possible increase in the membrane resting potentia