Assessment of Diosgenin Production by Trigonella foenum-graecum L. in vitro Condition

The objective of this study was to analyze and compare the accumulation of diosgenin during different stages of growth in leaf, shoot apical meristem and root apical meristem callus cultures of fenugreek. The levels of this secondary compound were examined by UV Spectrophotometer. The results showed that Callus generation increase was caused by the increased concentration of 2,4-D hormone. The highest callus generation was obtained in shoot apical meristem grown on 1 mg L-1 2,4-D hormone in 45 days was significant (p<0.05) compared with other treatments. We concluded that the level of diosgenin detected in leaf callus was more than its level in shoot and root callus.