Comparision of structural polypeptides of two field isolates of IBH virus

Inclusion Body Hepatitis (IBH) caused by adenovirus is a reemerging diseaseof mainly young broiler chicks of 3-5 weeks age. The polypeptide pattern of a local isolate of virus causing Inclusion Body Hepatitis was analyzed by sodium dodecyl slulphate-polyacrylamide gel electrophoresis. A total of 9 polypeptides ranging in molecular weight between 35.2 and 110.0 kDa were observed. Western blot analysis of structural polypeptides also revealed that all 9 polypeptides were immunogenic. Out of these 9 polypeptides, 2 viz 97.4 kDa and 53.9 kDa were found to be major immunogenic polypeptide based on intense staining. [Vet World 2009; 2(8.000): 301-303]