Examinations of efficacy of Peral-S under in vitro conditions

The examinations covered numerous clinical and referent strain of both bacteria, as well as viruses and fungi. The paper presents only one part of the examinations in which referent strains of E. coli NTCC 8116, Proteus mirabilis ATCC 19086, Staphylococcus aureus ATCC 511, Salmonella Choleraeuis ATCC 10653, Enterococcus faecalis ATCC 6055 and Bacillus cereus ATCC 11778 were used. The effect of Peral-S in concentrations of 0.25%, 0.5% and 1% on the listed strain of bacteria was examined using the suspension method at exposition times of 5, 10, 15 and 20 minutes. Examinations were also performed of the antiviral effects of Peral-S in concentrations of 0.1% to 1% and at exposure times from 30 seconds to 5 minutes on the viruses Aujeszky 257/II, P13, Newcastle virus, Coxackie B6 , Herpes simplex type I. The efficacy of the antiviral effects of Peral-S was checked on tissue cultures MDBK, AUBEK and HEP-2 using the floating technique method. Examinations also covered the fungicidal effect of Peral-S in concentrations of from 0.1% to 1%, and of 3%, 4%, and 5% at exposure times of 5, 15 and 60 minutes and cultures aged 1 to 14 days. The investigations covered fungi from the following genera: Penicillium spp., Aspergillus spp. and Mucor spp. Peral-S showed a bactericidal effect on all the examined bacteria strains, in all the applied concentrations and at all exposure times. Peral-S in a concentration of 0.1% and at an exposure time of 30 seconds had a vurcidal effect on all examined viruses. How-ever, there was no fungicidal effect of Peral-S in concentrations of 0.1% to 1% at all expo-sure times on fungi cultures aged from one to 14 days. The preparation did exhibit a fungicidal effect in a concentration of 3% in all examined fungi cultures after 60 minutes of expo-sure, while it exhibited a fungicidal effect in a concentration of 4% after 5 minutes of expo-sure on all examined fungi cultures, regardless of their age.