Prevalency of Klebsiella pneumoniae and producing Escherichia coli of β-lacta masas of widespread bogey (BLEE), in the Hospital San Jerónimo de Montería

One of the main problems at the Latin Americanhospitals has been the multiresistant microorganisms thatproduce ESBL. Objectives. To establish resistance phenotypicprofi le of Klebsiella pneumoniae (KP) and Escherichia coli(EC) that produce nosocomial infections at the San JeronimoHospital (HSJ) of Monteria, Colombia and to compare fourmethods for the detection of ESBL. Methods. 60 microorgan-isms, 30 KP and 30 EC isolated of nosocomial patients of HSJwere analyzed; disk diffusion of the National Committee forClinical Laboratory Standards (NCCLS) and the confi rmatorymethods for the detection of ESBL as combination of disk,Etest and MicroScan ESBL were used. Results. ESBLproducers were determined in 14 of 60 (23.3%) isolates, 11of 30 KP (36.6%) and 3 of 30 EC (10%). The disk diffusionprocedure recommended by NCCLS, MicroScan ESBL andEtest ESBL were in agreement about productions andconfi rmation of ESBL (p>0.05). The combined disk methodshowed differences when it was compared with the otherthree methods: the production of ESBL in 9 of 30 (30%) KPand 2 of 30 (6.6%) EC (p <0.05). The strains ESBL producerswere classifi ed in four resistance phenotypes. Conclusions.The study showed a high ESBL producers in KP and EC atthe HSJ. High frequency of ESBL found suggest to restrictthe broad spectrum Beta lactams and the utilization of strictlyhygiene measures for preventing the ESBL dissemination at intra-hospital level.