Comparative study of the antimicrobial efficiency of Neem leaf extract, Sodium hypochlorite and Biopure MTAD An in vitro study

AIM- The present study was aimed to evaluate alternative inexpensive, simple and effective method for the sanitization of the root canal system. The antimicrobial efficacy of neem leaf extract as irrigant was evaluated and compared with the standard irrigant sodium hypochlorite and Biopure MTAD. METHODOLGY-Neem leaf extract was prepared from neem leaves and pure ethanol. To check the antimicrobial efficacy of neem leaf extract, Biopure MTAD and 2.5% NaOCl, agar well diffusion method was performed. BHI agar plates were prepared and cultures were spread onto agar plates. The plates were incubated for 24 hours at 37?celsius aerobically. Following incubation the diameters of zone of bacterial inhibition (clear zone) were measured in millimetre. RESULTS- Biopure MTAD had the maximum antibacterial efficacy, exhibiting broader zones of inhibition and sodium hypochlorite the least. CONCLUSIONS - Under the conditions of this study Biopure MTAD offers maximum antibacterial antibacterial advantage over neem leaf extract and 2.5% NaOCl.