Development and implementation of the quality control panel of RT-PCR and real-time RT-PCR for avian influenza A (H5N1) surveillance network in mainland China

We developed a panel with lysates of seasonal influenza virus (H1N1, H3N2 and B), serials of diluted H5N1 virus lysates, and in-vitro transcribed H5 hemaglutinin (HA) and an artificial gene RNAs for RT-PCR and rRT-PCR quality control assessment. The validations of stability and reproducibility were performed on the panel. Additionally, the panel was implemented to assess the detection capability of Chinese human avian influenza networks.The panel has relatively high stability and good reproducibility demonstrated by kappa's tests. In the implementation of panel on Chinese human avian influenza networks, the results suggested that there were a relatively low number of discrepancies for both concise and reproducibility in Chinese avian influenza virus net works.A quality control panel of RT-PCR and real-time RT-PCR for avian influenza A (H5N1) surveillance network was developed. An availably statistical data, which are used to assess the detection capability of networks on avian influenza virus (H5N1), can be obtained relatively easily through implementation of the panel on networks.National and international efforts to enhance early disease detection and to increase diagnostic capacity have stimulated the formation of laboratory networks within and between public, animal, and even plant health areas. The success of these laboratory networks can be attributed to the implementation of standardized procedures and assays, specific training programs as well as a demonstrated proficiency samples. So far, avian influenza surveillance networks have formed for performance in many countries [1,2]. Highly pathogenic avian influenza virus (HPAIV) H5N1 continues to pose a significant threat to human health [3,4], although it remains a zoonotic infection [5,6]. A surveillance network with strong detection capability is required to detect any evidence that the virus has acquired the ability to transmit between humans or to emerge as the next pandemic strain.The Chinese influenza su