Przeciwgrzybicze w a ciwo ci glukozynolanów – minimalne st enie hamuj ce (MIC)

Introduction: Rapid increase of resistance of antropopathogenic fungi to routinely used antimycotic drugs forces researchers to search for new alternative methods of antifungal therapies. Among compounds investigated there are glucosinolates (GLS), whose antimycotic activity was proved against plant pathogens. Aim of the study: The aim of this study was to determine the value of minimal inhibition concentration (MIC) of GLS degradation products on yeast and yeast-like fungi isolated from human ontocenoses. Material and methods: Fungi were obtained from the collection of Department of Mycology, University of Warmia and Mazury in Olsztyn and isolated from the human respiratory system and digestive tract. GLS degradation products were originated from seeds of broccoli, small radish, mustard and cabbage and were prepared at the Department of Food Chemistry and Biodynamics of the Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences in Olsztyn using HPLC method. The MIC value was conducted with the disk-diffusion method. Results: Experiments showed that the strongest antifungal activity was demonstrated for GLS degradation products from broccoli (MIC reached 1.62±0.11 μmol/g), the lowest for GLS degradation products from small radish (MIC reached 5.87±4.08 μmol/g). Conclusions: The analysis of the inhibiting effect of GLS degradation products on the growth of fungi showed their possible usefulness for antimycotic therapy and prophylaxis.