PHYSICO CHEMICAL ANALYSIS OF TEXTILE EFFLUENT AND DECOLORIZATION OF TEXTILE AZO DYE BY BACILLUS ENDOPHYTICUS STRAIN VITABR13

The physicochemical characterization of the textile industry effluent collected from Coimbatore in Tamil Nadu,India was been carried out and the results showed high rates of temperature ( 40°C ), pH (7.51) and Electrical Conductivity (9565 μmhos/cm), Biological Oxygen Demand (275 mgl-1), Chemical Oxygen Demand (789 mgl-1), Total Suspended Solids (1750 mgl-1), Total Dissolved Solids (5875mg l-1 ), heavy metal ions, Total hardness ( Ca2+, Mg2+, Cl- & SO42- ) and colour over the prescribed fresh water limits. A potential bacterial strain(VITABR13) was isolated and selected from the textile effluent on the basis of rapid azo dye Acid Red 128(100mgl-1)decolorization and later identified as belonging to genus Bacillus based on Phenotypic characterization and phylogenetic analysis of the 16s rRNA gene sequence. Effects of physicochemical parameters (pH, Temperature, Carbon and Nitrogen sources) on the Acid Red 128 decolorization by the selected bacterium were studied. Decolorization was effective at pH 8, 35°C with starch and peptone as carbon and nitrogen sources and in static conditions. This decolorization potential increased the applicability of this microorganism for the dye removal.