THIAMINE AMELIORATE HEPATIC, RENAL DYSFUNCTION AND DYSLIPIDAEMIA IN DIABETIC RATS

This study was carried out to explore the effects of experimental diabetes mellitus (DM) and thiamine on liver and kidney functions as well as on blood glucose and lipid profiles in rats. Forty male wistar albino rats (200-230g) were assigned to four groups, (ten/group). The first group used as control group and injected i.p with citrate buffer (pH 4.5). The second group injected i.p by Streptozotocin, (STZ) at a single dosage of 45 mg/kg b. wt. dissolved in citrate buffer (pH 4.5). The third group was supplemented with Thiamine: (50 mg / kg diet) daily through out the experiment. The fourth group was injected with STZ as second group and after 10 days supplemented with thiamine as third group daily through out the experimental period. The experimental period was extended to sixty day. At the expiration of the 1st and 2nd month from starting of experiment, fasted control, diabetic control and diabetic treated rats were anesthetized under diethyl ether, blood samples were collected from the orbital venous sinus. The sera were separated and used for determining of glucose, total protein, albumin, AST, ALT, cholesterol, triacylglycerol, HDL-c, urea, uric acid and creatinine. The obtained data showed that DM caused marked liver and renal injuries reflected in decreased total protein, albumin and increased serum ALT, AST, urea, uric acid and creatinine as well as increased blood glucose level and lipid profiles. In contrast, thiamine additions results in amelioration of the most mentioned adverse effects. Thiamine can protect against diabetes induced hepatic, renal adverse effects as well as correction of dyslipidemia associated with diabetes mellitus.