Invitro regeneration of acorus Calamus – an important medicinal plant

Objective: The present study is an effort to identify suitable hormonal combinations for invitro propagation of Acorus calamus (L.).Materials & Methods: Inoculation of rhizome explants on MS media containing different combination and concentrations of auxins produces multiple shoots. Inoculation on MS media containing IBA alone produces number of roots. Plantlets produced using rootlets were acclimatized and transplanted.Result; About 90% of the in vitro Micropropagated plants were healthy and useful for propagation.Conclusion: In vitro preservation of germplasm is also safe method to protect species & reducing the risk of natural vagaries. Further estimation of secondary constituents from natural and in vitro plants and antimicrobial activity of both natural and invitro plants will be carried out in future.