Effect of fresh juice of Brassica oleracea Var. Capitata on isolated precontracted rat uterine horns

Plan: The present study was undertaken to investigate the H2 receptor antagonistic action of fresh juice of Brassica oleracea var. capitata Methodology: Two sets of experiments consisted of recording the responses of the uterine horn preparations (precontracted with potassium chloride, KCl) to histamine (4.5; 9; 17.99; 35.99; 71.98 × 10–4 and 14.40× 10–3 mol/L, 2 minute each concentration) in absence and presence of S- Chlorpheniramine, ranitidine and BOCJ. Relaxant responses were measured as changes in isometric tension and converted into percentage of the reference maximum relaxation induced by standard dose of histamine for each group of experiment.Outcome: Our results indicate that histamine (EC50 = 19.05 ± 1.76 × 10-4 mol/L, p < 0.001) produces relaxation of potassium chloride precontracted isolated rat uterus. This effect of histamine is abolished by ranitidine, a selective H2 histamine receptor antagonist and BOCJ in a concentration dependent manner.