Simple Spectrofluorimetric determination of Buspirone hydrochloride in Bulk drug and Pharmaceutical dosage forms.

Plan: An analytical method for estimation of Buspirone HCl in bulk drug and pharmaceutical dosage forms by spectrofluorimetric is described.Prologue: The method is simple, fast and accurate and can be used for routine analysis of commercial Buspirone HCl tablets.Methodology: The developed method is based on the determination of fluorescence intensity of the solutions in water at 379nm after excitation at 238nm. Different experimental parameters affecting the fluorescence were carefully studied. The fluorescence intensity of the solution was stable for 5 hours. The calibration curve was linear from 50-1500ng/ml. The limit of detection (LOD) and limit of quantification (LOQ) were 20ng/ml and 45ng/ml respectively. Outcome: The proposed method has been applied successfully for the determination of Buspirone HCl in pharmaceutical dosage forms. No significant interference was observed from excipients, coloring and flavoring agents commonly used in the formulation. The mean recovery of drug from tablets was 99.89%.