Method Development and validation of Levosalbutamol sulphate by Derivative Spectroscopy

Plan: Ananalytical method for estimation of levosalbutamol in bulk and pharmaceuticalformulation.Preface: The developedmethod is simple, fast and accurate and can be used for routine analysis ofMarket formulations.Methodology: In thisstudy zero, first and second order derivative spectrophotometric method weredeveloped for the estimation of Levosalbutamol sulphate. In zero orderspectrophotometery, absorbance value was measured at 277nm. In first derivativespectrophotometry amplitudes were measured at 233nm.In second derivativespectrophotometry amplitudes were measured at 237nm. Calibration curves werelinear between the concentration range of 20-60μg/ml, 5-30μg/ml and 35-60μg/mlrespectively. The % RSD value is less than 2% and the recovery were near 100%for all methods.Outcome: All the developed methods were applied on pharmaceutical formulations.Key words: Spectrophotometric method, Levosalbutamol sulphate, Derivativespectroscopy.