Simultaneous estimation of Azithromycin and Cefixime in Active Pharmaceutical Ingredients and Pharmaceutical dosage forms by Spectrophotometry

Plan: An analytical method for estimation of Azithromycin and Cefixime in bulk and tablet formulation.Methodology: A rapid, sensitive and specific uv-vis method was developed and validated for the estimation of Azithromycin and Cefixime in tablet dosage form. The method was validated in terms of linearity, accuracy, precision, specificity, limit of detection and limit of quantitation. The optimum conditions for the analysis of the drug were established. The maximum wavelength (λ max) of Azithromycin and Cefixime were found to be 235 nm and 288nm respectively. The percentage recovery of Azithromycin and Cefixime were 100.28-100.33 and 99.68-100.29 respectively. Beer’s law were obeyed in the concentration range of 10-50μg/ml for Azithromycin and 2-10μg/ml for Cefixime. The linear equation for Azithromycin and Cefixime were found to be y = 0.0187x - 0.0143, r2 = 0.9996 and y = 0.0917x + 0.026, r2 = 0.9985 respectively.Validation was performed as ICH guidelines for linearity, accuracy, precision, LOD and LOQ. Outcome: The proposed method was successfully applied for the quantitative determination of Azithromycin and Cefixime in tablet dosage form.