Localization of Ca2+ and Ca-ATPase on wet (Ruscus aculeatus) and dry (Primula officinalis) stigma surface

Calcium is present in the surface cells of both types of stigma. The chlorotetracycline method was used to show that the papillae of the dry stigma of Primula officinalis are the source of fluorescence of the CTC-Ca2+ complex. The precipitation method (NHA) localized calcium ions mainly in the pellicula surrounding these papillae. The NHA-Ca2+ precipitates were localized on the plasma membrane in the papillae of the wet stigma of Ruscus aculeatus. Ca-ATPase activity was found in both types of stigma in sites where the presence of Ca2+ had been determined.