Potential use of RAPD markers in characteristics of cucumber (Cucumis sativus L.) haploids and double-haploids

A study was designed to obtain hapolid and double haploid (DH) plants from cucumber cultivars tolerant to Pseudoperonospora cubensis. The main goal was to identify RAPD molecular markers associated with downy mildew resistance. On average, 20% of embryos generated in two experiments, were converted to haploid plants. RAPD markers that differentiated susceptible and resistant H and DH plants were identified. Somaclonal variation in DH lines was seldom detected.