Alterations in non-enzymatic antioxidant components of Catharanthus roseus exposed to paclobutrazol, gibberellic acid and Pseudomonas fluorescens

The effect of different plat growth regulators like paclobutrazol (PBZ) gibberellic acid and Pseudomonas fluorescens treatments on the non-enzymatic antioxidant components of Catharanthus roseus was investigated in the present study. The treatments were given to plants by soil drenching on 38, 53, 68 and 83 days after planting (DAP). The plants were taken randomly on 45, 60, 75 and 90 DAP and separated into root, stem and leaves and used for determining antioxidant potentials. The non-enzymatic antioxidant contents like total phenol, ascorbic acid (AA), reduced glutathione (GSH) and ?-tocopherol (?-toc), were extracted and assayed from both control and treated plants. It was found that paclobutrazol (PBZ) gibberellic acid and Pseudomonas fluorescens treatments have a profound effect on the antioxidant metabolism and caused an enhancement in non-enzymatic antioxidant potentials under treatments in Catharanthus roseus. Our results have good significance, as this increase the innate antioxidant potential of this medicinal plant, as this plant being an essential component of traditional as well as modern pharmaceutical systems.