THE EFFECT OF ASCORBIC ACID ON BLAST TRANSFORMATION OF LYMPHOCYTES

The impact of ascorbic acid on the intensity of blast transformation of lymphocytes induced by Con-A and LPS mytogens was considered. For the experiments the spleen lymphocytes C3H/HEJ mouse were used. The cells were explored to the impact of different ascorbic acid concentration, and the process of DNA synthesis was measured by frequent marking of 3H-TdR, which were incorporated into a newly sinthetisized DNA. The quantity of the incorporated 3H-TdR was measured by scintillation in the beta counter.The achieved results show that ascorbic acid exemplifies the stimulative influence on blast transformation lymphocyte in dose from 0,075 to 0,175 gamma/2,5x105 cells. With progressive increase of ascorbic acid dosage, the inhibitatory effect of ascorbic acid progressively increases. Ascorbic acid shows a stronger effect on T than on B lymphocytes. These results lead to a conclusion that ascorbic acid has an important effect on the intensity of stimulated lymphocyte activity by mitogen.