DYEING OF ELASTIC TISSUES WITHOUT DIFFERENTIATION: NEW, QUICKLY METHOD

Our methodological procedure established successful technique of the direct dying of elastic fibers and lamellas in elastic tissues. Experiments were carried out on a human autopsy material. Blocks rich in elastic fibers were fixed by 10% buffered neutral formaline and cut in the thickness of 5mm. Deparaffinization of slides is done by xylol treatment (2x15 min). Additional denaturation is achieved by combined solution, which contains 1.2 g of picric acid in 30% solution of glacial acetic acid. By chloramine B application as blocking agent in form of 1% DMSO solution the dye's affinity for eviromental tissue is decreased. Tissues prepared in this way are treated with 0.5% solution of acidic sulfonic color Evans Blue (C.I.23860) in ratio 2:1. Lamellas are dark violet with light violet periphery. Obtained results are in favor of high applicability of this method in light microscopy. In our opinion, this method can be recommended as one of the methods for identification of elastic tissues.